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death domain protein tradd polyclonal antibody  (Proteintech)


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    Proteintech death domain protein tradd polyclonal antibody
    Fig. 5. Astragalin (AST) ameliorated lipopolysaccharide (LPS)-induced acute liver injury (ALI) in mice by inhibiting necroptosis. (A-B) Western blotting measured the protein levels of tumor necrosis factor (TNF)-α, TANK-binding kinase 1 (TBK1), receptor-interacting protein kinase 1 (RIPK1), RIPK3, mixed lineage kinase domain-like protein (MLKL), and TNFR1-associated death domain protein <t>(TRADD).</t> The relative expression levels were normalized to β-actin. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, ns denotes no significance. (C-D) AST decreased the number of P-RIPK3 positive cells that was increased by LPS treatment. Student’s t-test was performed, n = 6, *** P < 0.001.
    Death Domain Protein Tradd Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/death+domain+protein+tradd+polyclonal+antibody/TRADD+Antibody/10__1016_slash_j__jff__2024__106298-60-96-125
    Average 93 stars, based on 18 article reviews
    death domain protein tradd polyclonal antibody - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism"

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism

    Journal: Journal of Functional Foods

    doi: 10.1016/j.jff.2024.106298

    Fig. 5. Astragalin (AST) ameliorated lipopolysaccharide (LPS)-induced acute liver injury (ALI) in mice by inhibiting necroptosis. (A-B) Western blotting measured the protein levels of tumor necrosis factor (TNF)-α, TANK-binding kinase 1 (TBK1), receptor-interacting protein kinase 1 (RIPK1), RIPK3, mixed lineage kinase domain-like protein (MLKL), and TNFR1-associated death domain protein (TRADD). The relative expression levels were normalized to β-actin. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, ns denotes no significance. (C-D) AST decreased the number of P-RIPK3 positive cells that was increased by LPS treatment. Student’s t-test was performed, n = 6, *** P < 0.001.
    Figure Legend Snippet: Fig. 5. Astragalin (AST) ameliorated lipopolysaccharide (LPS)-induced acute liver injury (ALI) in mice by inhibiting necroptosis. (A-B) Western blotting measured the protein levels of tumor necrosis factor (TNF)-α, TANK-binding kinase 1 (TBK1), receptor-interacting protein kinase 1 (RIPK1), RIPK3, mixed lineage kinase domain-like protein (MLKL), and TNFR1-associated death domain protein (TRADD). The relative expression levels were normalized to β-actin. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, ns denotes no significance. (C-D) AST decreased the number of P-RIPK3 positive cells that was increased by LPS treatment. Student’s t-test was performed, n = 6, *** P < 0.001.

    Techniques Used: Western Blot, Binding Assay, Expressing

    Related Articles

    Activity Assay:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.

    RNA Extraction:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.

    Lysis:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.

    Bicinchoninic Acid Protein Assay:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.

    cDNA Synthesis:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.

    Recombinant:

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism
    Article Snippet: We got assay kits from Nanjing Jiancheng Bioengineering Institute, including alanine aminotransferase assay kit (#C009-2–1), aspartate aminotransferase assay kit (#C010-2–1), malondialdehyde (MDA) assay kit (TBA method, #A003-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, #A001-1), total antioxidant capacity (T-AOC) assay kit (#A015-1), and catalase (CAT) assay kit (visible light, #A007-1–1). .. Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957). .. Other reagents, such as super ECL kit (#R230321 and #R230322) and horseradish enzyme labeled goat M.-M. Cao et al. Journal of Functional Foods 118 (2024) 106298 anti-rabbit IgG (H + L) (#ZB-2301 and #ZB-2305), were provided by Fengrbio Co. Ltd and Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, respectively.



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    Image Search Results


    Fig. 5. Astragalin (AST) ameliorated lipopolysaccharide (LPS)-induced acute liver injury (ALI) in mice by inhibiting necroptosis. (A-B) Western blotting measured the protein levels of tumor necrosis factor (TNF)-α, TANK-binding kinase 1 (TBK1), receptor-interacting protein kinase 1 (RIPK1), RIPK3, mixed lineage kinase domain-like protein (MLKL), and TNFR1-associated death domain protein (TRADD). The relative expression levels were normalized to β-actin. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, ns denotes no significance. (C-D) AST decreased the number of P-RIPK3 positive cells that was increased by LPS treatment. Student’s t-test was performed, n = 6, *** P < 0.001.

    Journal: Journal of Functional Foods

    Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism

    doi: 10.1016/j.jff.2024.106298

    Figure Lengend Snippet: Fig. 5. Astragalin (AST) ameliorated lipopolysaccharide (LPS)-induced acute liver injury (ALI) in mice by inhibiting necroptosis. (A-B) Western blotting measured the protein levels of tumor necrosis factor (TNF)-α, TANK-binding kinase 1 (TBK1), receptor-interacting protein kinase 1 (RIPK1), RIPK3, mixed lineage kinase domain-like protein (MLKL), and TNFR1-associated death domain protein (TRADD). The relative expression levels were normalized to β-actin. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, ns denotes no significance. (C-D) AST decreased the number of P-RIPK3 positive cells that was increased by LPS treatment. Student’s t-test was performed, n = 6, *** P < 0.001.

    Article Snippet: Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957).

    Techniques: Western Blot, Binding Assay, Expressing